Crystallization (Comment) | Organism |
---|---|
enzyme in complex with its acetyl-lysine 16 histone H4 substrate, nicotinamide and the reaction intermediate analog ADP-HPD, vapor diffusion method, using 2.0 M (NH4)2SO4, 100 mM Na citrate, pH 5.6, 200 mM K/Na tartrate or 2.0 M (NH4)2SO4 and 100 mM Na citrate, pH 5.5 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D118N | the mutant retains approximately 13% of the activity of the wild type protein | Saccharomyces cerevisiae |
I117A | inactive | Saccharomyces cerevisiae |
I117D | inactive | Saccharomyces cerevisiae |
I117F | the mutant is nearly as active as the wild type protein (about 75% activity) | Saccharomyces cerevisiae |
I117H | inactive | Saccharomyces cerevisiae |
I117L | the mutant retains approximately 25% of the activity of the wild type protein | Saccharomyces cerevisiae |
I117V | the mutant is nearly as active as the wild type protein (about 65% activity) | Saccharomyces cerevisiae |
I117W | inactive | Saccharomyces cerevisiae |
I117Y | inactive | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nicotinamide | noncompetitive inhibitor | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0161 | - |
NAD+ | wild type enzyme, at pH 8.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.0255 | - |
NAD+ | mutant enzyme I117V, at pH 8.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.0258 | - |
NAD+ | mutant enzyme I117F, at pH 8.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.4535 | - |
NAD+ | mutant enzyme D118N, at pH 8.0, temperature not specified in the publication | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53686 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fluorogenic acetyl-L-lysine + NAD+ + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
[histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O | - |
Saccharomyces cerevisiae | [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HST2 | - |
Saccharomyces cerevisiae |
Sir2 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |